Immunomagnetic microbeads for screening with flow cytometry and identification with nano-liquid chromatography mass spectrometry of ochratoxins in wheat and cereal

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Immunomagnetic microbeads for screening with flow cytometry and identification with nano-liquid chromatography mass spectrometry of ochratoxins in wheat and cereal

Multi-analyte binding assays for rapid screening of food contaminants require mass spectrometric identification of compound(s) in suspect samples. An optimal combination is obtained when the same bioreagents are used in both methods; moreover, miniaturisation is important because of the high costs of bioreagents. A concept is demonstrated using superparamagnetic microbeads coated with monoclona...

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Efficient Separations of Intact Proteins Using Slip-Flow with Nano-Liquid Chromatography–Mass Spectrometry

A capillary with a pulled tip, densely packed with silica particles of 0.47 μm in diameter, is shown to provide higher peak capacity and sensitivity in the separation of intact proteins by reversed-phase liquid chromatography-mass spectrometry (LC-MS). For a C18 bonded phase, slip flow gave a 10-fold flow enhancement to allow for stable nanospray with a 4-cm column length. Model proteins were s...

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Determination of deoxynivalenol and nivalenol in corn and wheat by liquid chromatography with electrospray mass spectrometry.

The fungus Fusarium graminearum is a pathogen of both wheat and corn. Strains of the fungus from the United States produce a toxin, deoxynivalenol (DON); strains of the fungus from Asia and Europe produce DON or a related toxin, nivalenol. These toxins can cause disease in livestock, and their potential presence in feed and foods is a concern for animal and human health. A method was developed ...

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ژورنال

عنوان ژورنال: Analytical and Bioanalytical Chemistry

سال: 2011

ISSN: 1618-2642,1618-2650

DOI: 10.1007/s00216-011-4974-7